CM-0001

ASP -12 (Artificial Seawater Medium) McLachlan, 1973; modified according to McFadden & Melkonian, 1986.

Seawater

CM-0001 [ASP-12]
No es un medio concentrado
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55,00 €

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# Component Stock solution (g . L-1 dH2O) Addition per 1 Litre culture medium
1 HEPES 238.10 g 3 ml
2 NaCl 28 g
3 KCl 60.00 g 11.6 ml
4 MgSO4 . 7 H2O 7 g
5 MgCl2 . 6 H2O 4 g
6 CaCl2 . 2 H2O 370.00 g 4 ml
7 NaNO3 100.30 g 1 ml
8 K3PO4 . 3 H2O 12.50 g 1 ml
9 Na2-Glycerophosphate 6.85 g 1 ml
10 Na2SiO3 . 9 H2O 28.42 g 5.3 ml
11 NTA (Titriplex I)* 10.00 g 10 ml
12 Vitamins 1 ml
13 Trace Metals 1 ml
14 Soil Extract 10 ml

Preparation of Vitamins Solution

# Component Stock solution concentration Addition per 1 Litre stock solution
1 Vitamin B12 0.20 mg
2 Biotin 1.00 mg
3 Thiamine-HCl 100.00 mg
4 Niacinamide 0.10 mg

Preparation of Trace Metal Solution

10g of garden-soil is mixed with 120 ml bidistilled water and boiled for 10 minutes. Afterwards it is centrifuged for 10 minutes (low speed), and the supernatant is filtered through a series of membrane filters from 1.2 μm – 0.1 μm pore size. The remaining filtrate is adjusted to 100 ml with bidistilled water. Aliquots of 10 ml are stored frozen.
The soil should not be recently fertilized and should not contain too much humus.

# Component Stock solution (g . L-1) Addition per 1000 ml bidistilled water
1 Na2EDTA .2 H2O 4.36 g
2 FeCl3 . 6 H2O 3.15
3 K2CrO4 1.94 1 ml
4 CoCl2 . 6 H2O 11.90 1 ml
5 CuSO4 . 5 H2O 2.50 1 ml
6 MnCl2 . 4 H2O 178.10 1 ml
7 Na2MoO4 . 2 H2O 19.90 1 ml
8 NiSO4 . 6 H2O 2.63 1 ml
9 H2SeO3 1.29 1 ml
10 Na3VO4 1.84 1 ml
11 ZnSO4 . 7 H2O 23.00 1 ml

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.9 with NaOH and autoclave.

* Titriplex I - Nitrilotriacetic acid - C6H9NO6


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