CM-0022

Bold's Basal Medium with additional Vitamins Bold 1949, Bischoft & Bold 1963.

Freshwater

CM-0022 [BBM + V]
No es un medio concentrado
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55,00 €

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# Component Final concentration Stock solution (g . L-1 dH2O) Addition per 1 Litre culture medium
1 NaNO3 2,94 mM 25.00 10 ml
2 CaCl2 . 2H2O 0.17 mM 2.50 10 ml
3 MgSO4 . 7H2O 0.3 mM 7.50 10 ml
4 K2HPO4 0.43 mM 7.50 10 ml
5 KH2PO4 1.29 mM 17.50 10 ml
6 NaCl 0,43 mM 2.50 10 ml
7 Alkaline EDTA solution
EDTA (Titriplex III)
KOH		 17.10 mM
55.30 mM
50 g
31 g
1 ml
8 Acidified Iron solution
FeSO4 . 7 H2O
H2SO4		 0.179 mM
4.98
1 ml
1 ml
9 Boron solution
H3BO3		 18.50 mM 11.42
1 ml
10 Vitamins solution see below 3 ml
11 Trace Metals solution
ZnSO4 .7H2O
MnCl2 .4H2O
MoO3
CuSO4 .5H2O
Co(NO3)2 .6H2O		 8.82
1.44
0.71
1.57
0.49		 0.307 µM
7.28 µM
4.93 µM
6.29 µM
1.68 µM



1ml

Preparation of Vitamins Solution

# Vitamin Final concentration Stock solution concentration Addition per 1 Litre stock solution
1 Vitamin B12 0.15 nM 0.20 mg
2 Biotin 4.10 nM 1.0 mg
3 Thiamine-HCl 0.30 µM 100.00 mg
4 Niacinamide 0.80 nM 0.10 mg

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. The final pH should be 6.6.


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