CM-0014

McFadden & Melkonian, 1986.

Freshwater

CM-0014 [Waris-H]
No es un medio concentrado
Ver detalles

55,00 €

Añadir al carrito

# Component Stock solution Addition per 1 Litre stock solution Addition per 1 Litre culture medium
1 KNO3 100.00 g 1 ml
2 MgSO4 . 7H2O 20.00 g 1 ml
3 (NH4)2HPO4 20.00 g 1 ml
4 Ca(NO3)2 . 4
H2O		 100.00 g 1 ml
5 HEPES 238.31 g 1 ml
6 P-II Metals 1 ml
7 Fe-EDTA 1 ml
8 Vitamins 1 ml
9 Soil extract 10 ml

Preparation of PII Metals Solution

# Component Stock solution Addition per 1 Litre stock solution
1 EDTA (Titriplex III)* 3.00 g
2 H3BO3 1.14 g
3 MnCl2 . 4 H2O 0.144 g
4 ZnSO4 . 7 H2O 0.021 g
5 CoCl2 . 6 H2O 0.004 g

Preparation of Fe-EDTA Solution

# Component Stock solution concentration Addition per 1 Litre stock solution
1 EDTA (Titriplex II)* 5.22 g
2 FeSO4 . 7 H2O 4.98 g
3 1 N KOH 54.00 ml

EDTA (Titriplex II) and FeSO4 x 7 H2O is heated for 30 min (100oC); KOH is added to the cooled mixture

Preparation of Vitamins Solution

Preparation of Soil Extract

10g of garden-soil is mixed with 120 ml bidistilled water and boiled for 10 minutes. Afterwards it is centrifuged for 10 minutes (low speed), and the supernatant is filtered through a series of membrane filters from 1.2 µm – 0.1 µm pore size. The remaining filtrate is adjusted to 100 ml with bidistilled water. Aliquots of 10 ml are stored frozen. The soil should not be recently fertilized and should not contain too much humus.

# Vitamin Stock solution concentration Addition per 1 Litre stock solution
1 Vitamin B12 0.20 mg
2 Biotin 1.00 mg
3 Thiamine-HCl 100.00 mg
4 Niacinamide 0.10 mg

Preparation of Culture Solution

Add 1 ml of stock solutions 1-8 to 1000 ml of bidistilled water. Add 10 ml of thawed soil extract (= stock solution 9), adjust the pH to 7.0 and autoclave.

* Titriplex II - Ethylenediaminetetraacetic acid - C10H16N2O8
* Titriplex III - Ethylenediaminetetraacetic acid disodium salt dihydrate - C10H14N2Na2O8 x 2 H2O


Volver al listado