CM-0004

Bold´s Basal Medium Bold 1949, Bischoft & Bold 1963.

CM-0004 [BBM]
No es un medio concentrado

#	Component	Stock solution (g . L-1 dH2O)	Addition per 1 Litre culture medium
1	NaNO₃	25.00	10 ml
2	CaCl₂ . 2H₂O	2,50	10 ml
3	MgSO₄ . 7H₂O	7.50	10 ml
4	K₂HPO₄	7.50	10 ml
5	KH₂PO₄	17.50	10 ml
6	NaCl	2,50	10 ml
7	Alkaline EDTA solution EDTA (Titriplex III) KOH	50 31	1 ml
8	Acidified Iron solution FeSO₄ . 7 H₂O H₂SO₄	4.98 1 ml	1 ml
9	Boron solution H₃BO₃	11.42	1 ml
10	Trace Metals solution ZnSO₄ .7H₂O MnCl₂ .4H₂O MoO₃ CuSO₄ .5H₂O Co(NO₃)₂ .6H₂O	8.82 1.44 0.71 1.57 0.49	1 ml

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. The final pH should be 6.6.