CM-0002

ASP-12 Medium with 2-fold NaCl McLachlan, 1973; modified according to McFadden & Melkonian, 1986.

Hypersaline

CM-0002 [ASP-12 + 2 x NaCl]
No es un medio concentrado
Ver detalles

55,00 €

Añadir al carrito

Medium used to culture Dunaliella sps. Prepare ASP-12 culture medium as indicated below, (according to J. McLachlan: Growth media – marine. In: Handbook of Phycological Methods, Culture Methods and Growth Measurements; ed.: Janet R. Stein; pp. 25 – 52) however, add 58 g of NaCl.

# Component Stock solution (g . L-1 dH2O) Addition per 1 Litre culture medium
1 HEPES 238.10 g 3 ml
2 NaCl 56 g
3 KCl 60.00 g 11.6 ml
4 MgSO4 . 7 H2O 7 g
5 MgCl2 . 6 H2O 4 g
6 CaCl2 . 2 H2O 370.00 g 4 ml
7 NaNO3 100.30 g 1 ml
8 K3PO4 . 3 H2O 12.50 g 1 ml
9 Na2-Glycerophosphate 6.85 g 1 ml
10 Na2SiO3 . 9 H2O 28.42 g 5.3 ml
11 NTA (Titriplex I)* 10.00 g 10 ml
12 Vitamins 1 ml
13 Trace Metals 1 ml

Preparation of Vitamins Solution

# Component Stock solution concentration Addition per 1 Litre stock solution
1 Vitamin B12 0.20 mg
2 Biotin 1.00 mg
3 Thiamine-HCl 100.00 mg
4 Niacinamide 0.10 mg

pH of this solution should be around pH 7.0

Preparation of Trace Metal Solution

# Component Stock solution (g . L-1) Addition per 1000 ml bidistilled water
1 Na2EDTA . 2 H2O 4.36 g
2 FeCl3 . 6 H2O 3.15
3 K2CrO4 1.94 1 ml
4 CoCl2 . 6 H2O 11.90 1 ml
5 CuSO4 . 5 H2O 2.50 1 ml
6 MnCl2 . 4 H2O 178.10 1 ml
7 Na2MoO4 . 2 H2O 19.90 1 ml
8 NiSO4 . 6 H2O 2.63 1 ml
9 H2SeO3 1.29 1 ml
10 Na3VO4 1.84 1 ml
11 ZnSO4 . 7 H2O 23.00 1 ml

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.9 with NaOH and autoclave.

* Titriplex I - Nitrilotriacetic acid - C6H9NO6


Volver al listado