CM-0013

Spirulina Medium Aiba & Ogawa, 1977, modified.

CM-0013 [Sp]
No es un medio concentrado

#	Component	Stock solution	Addition per 1 Litre culture medium
1	Solution I NaHCO₃ Na₂CO₃ K₂HPO₄	500 ml	13.61 g 4.03 g 0.50 g
2	Solution II NaNO₃ K₂SO₄ NaCl MgSO₄ · 7 H₂O CaCl₂ · 2 H₂O FeSO₄ · 7 H₂O Na₂EDTA · 2H₂O Trace metals solution	500 ml	2.5 g 1.0 g 1.0 g 0.2 g 0.04 g 0.01 g 0.08 g 5 ml
3	Vitamins solution		1 ml

#	Component	Stock solution (g 100 ml-1 dH2O)	Addition per 1 Litre final stock solution
1	Na₂EDTA · 2 H₂O		0.8 g
2	FeSO₄ · 7 H₂O		0.7 g
3	ZnSO₄ · 7 H₂O	0.1	1 ml
4	MnSO₄ · 7 H₂O	0.1	2 ml
5	H₃BO₃	0.2	5 ml
6	Co(NO₃)₂ · 6 H₂O	0.02	5 ml
7	Na₂MoO₄ · 2 H₂O	0.02	5 ml
8	CuSO₄ · 5 H₂O	0.0005	1 ml

Dissolve the EDTA into 900 mLof dH₂O and then dissolve each remaining component; bring the final volume to 1 liter.

#	Vitamin	Stock solution concentration Addition per 1 Litre stock solution
1	Vitamin B₁₂	0.20 mg
2	Biotin	1.00 mg
3	Thiamine-HCl	100.00 mg
4	Niaciamide	0.10 mg

Autoclave Solutions I and II separately and cool. Aseptically combine the two Solutions.