CM-0003

ASP -H Medium (Artificial Brackish Water) McLachlan, 1973; modified according to McFadden & Melkonian, 1986.

Brackishwater

CM-0003 [ASP-H]
No es un medio concentrado
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55,00 €

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# Component Stock solution (g . L-1 dH2O) Addition per 1 Litre culture medium
1 NaCl 18 g
2 MgSO4 . 7 H2O 5 g
3 KCl 60.00 g 10 ml
4 HEPES 238.31 g 3 ml
5 CaCl2 . 2 H2O 370.00 g 1 ml
6 NaNO3
+ K2HPO4 . 3 H2O
+ Na2CO3		 50.00 g
5.00 g
30.00 g		 1 ml
7 NTA (Titriplex I)*
NTA dissolves after addition of NaOH pellets and heating		 10.00 g 1 ml
8 Fe-EDTA solution 1 ml
9 Trace Metals 1 ml
10 H3BO3 2.97 g 10 ml
11 Vitamins solution 1 ml

Preparation of Fe-EDTA Solution

# Component Stock solution concentration Addition per 1 Litre stock solution
1 EDTA (Titriplex III)* 5.22 g
2 FeSO4 . 7 H2O 4.98 g
3 1 N KOH 54.00 ml

EDTA (Titriplex II) and FeSO4 x 7 H2O is heated for 30 min (100oC); KOH is added to the cooled mixture.

Preparation of Trace Metal Solution

# Component Stock solution concentration Addition per 1 Litre stock solution
1 ZnCl2 0.33 g
2 MnCl2 . 4 H2O 4.30 g
3 CoCl2. 6 H2O 0.12 g
4 Na/EDTA (Titriplex III)* 25.80 g

pH of Trace Metal Solution is adjusted to 7.5 with 10 N NaOH

Preparation of Vitamins Solution

# Component Stock solution concentration Addition per 1 Litre stock solution
1 Vitamin B12 0.20 mg
2 Biotin 1.00 mg
3 Thiamine-HCl 100.00 mg
4 Niacinamide 0.10 mg

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.7 with NaOH and autoclave.
* Titriplex I - Nitrilotriacetic acid - C6H9NO6
* Titriplex II - Ethylenediaminetetraacetic acid - C10H16N2O8

*Titriplex III - Ethylenediaminetetraacetic acid disodium salt dihydrate
- C10H14N2Na2O8 x 2 H2O


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