CM-0009
| # | Component | Stock solution (g . L-1 dH2O) | Addition per 1 Litre culture medium | |
|---|---|---|---|---|
| 1 | NaNO3 | 75 g | 1 ml | |
| 2 | NaH2PO4 . 2 H2O | 5 g | 1 ml | |
| 3 | Trace Metals | 1 ml | ||
| 4 | Vitamins | 0.5 ml |
Preparation of Trace Metal Solution
| # | Component | Stock solution (g . L-1) | Addition per 1000 ml bidestilled water | |
|---|---|---|---|---|
| 1 | Na2EDTA . 2 H2O | 4.36 g | ||
| 2 | FeCl3 . 6 H2O | 3.15 | ||
| 3 | K2CrO4 | 1.94 | 1 ml | |
| 4 | CoCl2 . 6 H2O | 11.90 | 1 ml | |
| 5 | CuSO4 . 5 H2O | 2.50 | 1 ml | |
| 6 | MnCl2 . 4 H2O | 178.10 | 1 ml | |
| 7 | Na2MoO4 . 2 H2O | 19.90 | 1 ml | |
| 8 | NiSO4 . 6 H2O | 2.63 | 1 ml | |
| 9 | H2SeO3 | 1.29 | 1 ml | |
| 10 | Na3VO4 | 1.84 | 1 ml | |
| 11 | ZnSO4 . 7 H2O | 23.00 | 1 ml |
Preparation of Vitamins Solution
| # | Vitamin | Stock solution (g . L-1) | Addition per 1 Litre stock vitamin solution | |
|---|---|---|---|---|
| 1 | Biotin | 0.5 | 1 ml | |
| 2 | Thiamine-HCl | 100 mg | ||
| 3 | Cyanocobalamin | 0.5 | 1 ml |
Add chemicals and stock solutions as indicated to 950 ml of filtered natural seawater and bring to final volume to 1 liter using filter natural seawater. Adjust pH to 8 with 1M NaOH.
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